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Preparation of Standard Gradient Working Solution: Add 1 mL of Universal Diluent to the lyophilized standard
Platelets and endothelial cells are among the first cells to respond during the development of sepsis
and HRP conjugate are sequentially added to microwells pre-coated with Chemokine CXC-Motif Receptor 4 (CXCR4) capture antibody
followed by 50 μl of Color Developing Reagent B
forming a double β-barrel assembly with six protruding coiled coils
Human M-CSF ELISA Kit ELISA Kit Preparation of Standard Gradient WorkingProduct Specification Usage Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or 4 C overnight, then centrifuge at 1000 g for 20 minutes, take the supernatant, or store the supernatant at 20 C or 80 C, but avoid repeated freezing and thawing. Plasma: Collect specimens with EDTA or heparin as anticoagulants, and centrifuge the specimens at 2 8 1000 g for 15
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