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Widhopf GF 2nd
incubation time and temperature of Proteinase K need to be optimized according to different types of tissue samples
CD73 protects endothelial barrier function and inhibits leukocyte trafficking
each cell sample should have up to a few 500 cells
Pipette 500uL of the 1000U/L standard working solution into the first EP tube and mix thoroughly to make a 500U/L standard working solution
Human GPRC5A ELISA Kit Size:96T Widhopf GF 2ndProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and